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THE "LITTLE BODIES       1            93

      ments. Whole healthy grapes, with their stalks attached,
      were introduced direct from the vine into boiled sweetened
      water, cooled in a current of carbonic acid gas, while the
      gas still bubbled into the liquid. Fermentation took place
      and was completed in this medium, preserved during the
      whole process from the influence of air. The same experi-
      ment succeeded when the grapes were introduced into
      must, filtered, heated and creosoted.
        From these researches it was evident that neither oxygen
      nor air-borne organisms were the cause of the fermenta-
      tion, but that the grape carried with it the provocative
      agents.
        Professor Bechamp communicated the    results of his
      experiments to the Academy of Science in   1864, and
      among its Reports the subject may be found exhaustively
      treated.  1  He had come to the conclusion that the agent
      that causes the must to ferment is a mould that comes
      from the outside of the grape, and that the stalks of grapes
      and the leaves of vines bear organisms capable of causing
      both sugar and must to ferment; moreover, that the fer-
      ments borne on the leaves and stalks are sometimes of a
      kind to injure the vintage.
        The year 1864, when Bechamp presented this Memoir,
      marks an era in the history of biological research, for, on
      the 4th April, of that self-same year, he read before the
      Academy of Science, his explanation of the phenomena of
      fermentation. He showed the latter to be due to the pro-
      cesses of nutrition of living organisms, that absorption
      takes place, followed by assimilation and excretion and
      for the  first time used the word zymase to designate a
      soluble ferment.
        It was the following year that M. Duclaux, a pupil of
      Pasteur's, tried to cast scorn upon Bechamp's illuminating
      explanation, thus supplying documentary proof that his
      master had no right to lay claim to having been a pioneer
      of this teaching.
        Bechamp, who, in 1857, had so conclusively proved air-
       1
        Comptes Rendus 59, p. 626.
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